科研动态

■ 科研方向

趋化因子受体拮抗剂的抗感染研究
建立人类附睾cDNA文库筛选特异性表达基因及其功能研究

■ 承担课题

1 、细胞因子受体拮抗剂的抗感染研究，与加拿大萨斯卡切温大学JOHN GORDON教授合作，提供部分资金2万加元。
2 、参与一项山东省科技厅资助的山东省科学技术攻关计划项目( 项目起止时间:2003.7-2006.12): 建立人类附睾cDNA文库筛选特异性表达基因及其功能研究(项目编号:032050102).目前程桂芝副教授在该实验室完成其博士论文。
3 、人高亲和力 CXCR1/2 全新拮抗剂的构建及其抗炎症研究 国家自然基金两项2005-2006，2008-2010
4 、国家人事部回国人员启动基金 一项

■ 学术交流
1. 参加2005年4月17日~22日，中国杭州，国际免疫学术会议，并作报告。
2. 2005年4月邀请加拿大萨斯卡切温大学微生物系免疫学研究室教授JOHN.R.GORDEN来大连医科大学做访问。
■ 2001年以来发表部分文章
1- F. Li, X. Zhang, C. Mizzi, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes the neutrophil chemoattractants present in pasteurellosis and mastitis lesions and abrogates neutrophil influx into intradermal endotoxin challenge sites in vivo. Veterinary Immunology and Immunopathology 90:65-77
2- W. Zhang, Z. Chen, F. Li, H. Kamencic, B. Juurlink, J.R. Gordon, and J. Xiang. 2002. TNF transgene-expressing dendritic cells undergo augmented cellular maturation, and induce more robust T cell activation and antitumor immunity than dendritic cells generated in recombinant TNF. Immunology 108:177-88.
3- Li, F., X. Zhang, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes ligand binding to the CXCR1 and CXCR2, and neutrophil responses to CXCL8/IL-8. Biochemical and Biophysical Research Communications 293:939-44
4- H Huang, F Li, JR Gordon, and J Xiang. 2002. Synergistic enhancement of anti-tumor immunity with adoptively transferred tumor-specific CD4+ and CD8+ T cells and intratumoral lymphtactin transgene expression. Cancer Research 62:2043-51
5- A.M. Schneider, X. Zhang, F. Li, and J.R. Gordon. 2001. Differential induction of allergen-specific IgA, airway hyperresponsiveness, or allergic airway disease following sensitization with limiting doses of ovalbumin-alum. Cellular Immunology 212:101-109
6- F. Li and J.R. Gordon. 2001. IL-8(3-73)K11R is a high affinity agonist of the neutrophil CXCR1 and CXCR2. Biochemical and Biophysical Research Communications 286:595-600
7- C.M.Cairns, J.R.Gordon, F.Li, & J. Xiang. 2001. Lymphotactin expression by engineered myeloma tumor cells drives tumor regression: Mediation by CD4+ and CD8+ T cells and neutrophils expressing the XCR1 receptor. Journal of Immunology 167: 57-65
8- H Huang, F Li, CM. Cairns, JR Gordon, and J Xiang. 2001. Neutrophils and B lymphocytes express the XCR1 receptor and chemotactically respond to lymphotactin. Biochemical Biophysical Research Communications 281:378-82.
9. Gordon JR, Li F. 2002. bIL-8 (3-73)K11R/G31P antagonizes human neutrophil responses to ELR-CXC chemokines, as well as in vivo bacterial endotoxin (LPS)-induced inflammatory responses in cattle. FASEB Journal, 16:A1079-1080
10. Li Fang , Mo Xiaoning, Zhang Yingmei, et al. 1999. Studies on a mutant human granulocyte colony-stimulating factor and its bioactivity both in vitro and in vivo. Chin Micorbiol Immunol 19(4) 319-322
11. Li Fang, Deng Qingdong et al. 2002. The effects of space aeronautical condition on the genetic and biological characteristics of Lactobacilli. Chin J Microecol 14(3) 14-15
12 J.R. Gordon, X. Zhang, F. Li, and A. Nayyar. 2003. Protection against endotoxin-induced pulmonary pathology (neutrophilic inflammation & lung function) by systemic delivery of the CXCR1/CXCR2 antagonist CXCL8(3-73)K11R/G31P. FASEB Journal, 17:C46
13. Weidong Zhang, Zhuang Chen, Fang Li, Huse Kamencic, Bemie,Juurilink John,R Gordon, Jim Xiang. TNF transgene-expressing dendritic cells undergo augmented cellular maturation, and induce more robust T cell activation and antitumor immunity than dendritic cells generated in recombinant TNF. Immunology, 18(2)117 2003
14．杨高云，狄春辉，蒋明，李芳，张颖妹，袁岚，宋泉声，马大龙。2002。白介素I 受体拮抗剂基因治疗小鼠Ⅱ型胶原关节炎的疗效观察。中华医学杂志，82（14）990-994
15 J.R. Gordon, V.A. Swystun, F Li, X Zhang, B.E. Davis, P Hull and D.W. Cockcroft. 2003 Regular salbutamol use increases CXCL8 responses in asthma. Relationship to the eosinophil response. European Respiratory Journal， 22: 118–126
16.F. Li and J.R.Gordon. 2001 IL-8(3-73)K11R/G31P is a High-Affinity Antagonist of the CXCRI and CXCRII, and Efficiently Antagonizes Neutrophil responses to Chemoattractants Expressed in Bacterial Pneumonia and Mastitis. FASEB J vol 14
17. A.M. Schneider, X. Zhang, F. Li, and J.R. Gordon. 2001. Differential induction of allergen-specific IgA, airway hyperresponsiveness, or allergic airway disease following sensitization with limiting doses of ovalbumin-alum. FASEB J vol 14
18. J.R. Gordon, X. Zhang, F. Li, and A.Nayyar. 2003. therapeutic or prophylactic delivery of the CXCR1/CXCR2 antagonist IL-8(3-73)K11R/G31P protects against endotoxin-induced acute respiratory distress. Inflammation Research 52:S164
19 JR Gordon1, F Li, A Nayyar, J Xiang, and X Zhang . 2005 CD8+, but not CD8-, Dendritic Cells Tolerize Th2 Responses Via Contact-Dependent and-Independent Mechanisms, and Reverse Airway Hyperresponsiveness, Th2,and Eosinophil Responses in a mouse Model of Asthma, The Journal of Immunology,175:1516-1522
20 Yongqing Liu, Dajing Xia, Fang Li, Changyu Zheng, and Jim Xiang. 2005 Intratumoral administration of immature dendritic cellsfollowing the adenovirus vector encoding CD40 ligand elicits
significant regression of established myeloma. Cancer Gene Therapy 12, 122–132
21 Dajing Xia, Fang Li, Changyu Zheng, and Jim Xiang. 2004 Engineered Fusion Hybrid Vaccine of IL-18 Gene-Modified Tumor Cells and Dendritic Cells Induces Enhanced Antitumor Immunity
Cancer Biotherapy & Radiopharmaceuticals 19(3): 322-330
22．John R. Gordon, Fang Li, Xiaobei Zhang, Wenjun Wang, Xixing Zhao, and Aarti Nayyar 2005 The combined CXCR1/CXCR2 antagonist CXCL8(3–74)K11R/G31P blocks neutrophil infiltration, pyrexia, and pulmonary vascular pathology in endotoxemic animals Journal of Leukocyte Biology 78：1265
23 JR Gordon1, F Li, A Nayyar, J Xiang, and X Zhang . 2005 CD8+, but not CD8-, Dendritic Cells Tolerize Th2 Responses Via Contact-Dependent and-Independent Mechanisms, and Reverse Airway Hyperresponsiveness, Th2,and Eosinophil Responses in a mouse Model of Asthma, The Journal of Immunology,175:1516-1522
24 Yongqing Liu, Dajing Xia, Fang Li, Changyu Zheng, and Jim Xiang. 2005 Intratumoral administration of immature dendritic cells following the adenovirus vector encoding CD40 ligand elicits significant regression of established myeloma. Cancer Gene Therapy 12, 122–132
25 Dajing Xia, Fang Li, Changyu Zheng, and Jim Xiang. 2004 Engineered Fusion Hybrid Vaccine of IL-18 Gene-Modified Tumor Cells and Dendritic Cells Induces Enhanced Antitumor Immunity Cancer Biotherapy & Radiopharmaceuticals 19(3): 322-330
26 John R. Gordon, Fang Li, Xiaobei Zhang, Wenjun Wang, Xixing Zhao, and Aarti Nayyar 2005 The combined CXCR1/CXCR2 antagonist CXCL8(3–74)K11R/G31P blocks neutrophil infiltration, pyrexia, and pulmonary vascular pathology in endotoxemic animals Journal of Leukocyte Biology 78：1265